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klb  (R&D Systems)


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    Structured Review

    R&D Systems klb
    Klb, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti%E2%80%91klb/Human+Klotho+beta+Antibody/pm41752116-173-8-14
    Average 94 stars, based on 15 article reviews
    klb - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Feto-maternal cholesterol transport regulated by β-Klotho–FGF15 axis is essential for fetal growth
    Article Snippet: .. After blocking with 5% skim milk in TBS-T for 1 h at room temperature, the membrane was incubated overnight with goat anti-mouse KLB polyclonal antibody (AF2619, 1:1,000; R&D Systems) at 4°C. ..

    Article Title: Feto-maternal cholesterol transport regulated by β-Klotho-FGF15 axis is essential for fetal growth.
    Article Snippet: .. After blocking with 5% skimmilk in TBS-T for 1 h at room temperature, the membrane was incubated overnight with goat anti-mouse KLB polyclonal antibody (AF2619, 1:1,000; R&D Systems) at 4°C. ..

    Membrane:

    Article Title: Feto-maternal cholesterol transport regulated by β-Klotho–FGF15 axis is essential for fetal growth
    Article Snippet: .. After blocking with 5% skim milk in TBS-T for 1 h at room temperature, the membrane was incubated overnight with goat anti-mouse KLB polyclonal antibody (AF2619, 1:1,000; R&D Systems) at 4°C. ..

    Article Title: Feto-maternal cholesterol transport regulated by β-Klotho-FGF15 axis is essential for fetal growth.
    Article Snippet: .. After blocking with 5% skimmilk in TBS-T for 1 h at room temperature, the membrane was incubated overnight with goat anti-mouse KLB polyclonal antibody (AF2619, 1:1,000; R&D Systems) at 4°C. ..

    Incubation:

    Article Title: Feto-maternal cholesterol transport regulated by β-Klotho–FGF15 axis is essential for fetal growth
    Article Snippet: .. After blocking with 5% skim milk in TBS-T for 1 h at room temperature, the membrane was incubated overnight with goat anti-mouse KLB polyclonal antibody (AF2619, 1:1,000; R&D Systems) at 4°C. ..

    Article Title: Feto-maternal cholesterol transport regulated by β-Klotho-FGF15 axis is essential for fetal growth.
    Article Snippet: .. After blocking with 5% skimmilk in TBS-T for 1 h at room temperature, the membrane was incubated overnight with goat anti-mouse KLB polyclonal antibody (AF2619, 1:1,000; R&D Systems) at 4°C. ..

    other:

    Article Title: Cancer-associated adipocytes mediate CD8 + T cell dysfunction via FGF21-driven lipolysis
    Article Snippet: The following antibodies were used: anti-FGFR1 (cat#9740, CST, 1:1000); PhosphoFGFR1 (cat#52928, CST, 1:1000); anti-KLB (cat#AF2619, R&D, 1:1000); p38 MAPK (D13E1) (cat#8690, CST, 1:1000); Phospho-p38 MAPK (Thr180/ Tyr182) (cat#4511, CST, 1:1000); β-Actin (cat#S0B0074, STARTER, 1:1000); ATGL (cat#2138, CST, 1:1000); HRP-Rabbit anti-Goat (cat#AS029, Abclonal, 1:20000); HRP Goat anti-Rabbit IgG (H+L) (cat#AS014, Abclonal,1:20000).

    Article Title: Sodium sulphate ameliorates hypercholesterolemia via the upregulation of Cyp7a1 in hepatocytes and alleviates hepatic insulin resistance via the downregulation of Trib3 in mice with high cholesterol diets.
    Article Snippet: The primary antibodies comprised: Rabbit anti‐GaPDH (1:1,000; cat. no. 2118S; CST Biological Reagents Co., Ltd.), mouse anti‐CYP7A1 (1:1,000; cat. no. 2683295; MilliporeSigma), rabbit anti‐hydroxy‐δ‐5‐steroid dehydrogenase, 3 β‐ and steroid δ‐isomerase 7 (HSD3B7; 1:1,000; cat. no. ab190223; abcam), rabbit anti‐aldo‐keto reductase family 1 member D1 (aKR1D1; 1:1,000; cat. no. ab101393; abcam), rabbit anti‐acyl‐Coa oxidase 2 (1:1,000; cat. no. ab197808; abcam), rabbit anti‐hydroxysteroid 17‐β dehydrogenase 4 (HSD17B4; 1:1,000; cat. no. ab97971; abcam), rabbit anti‐CYP27a1 (1:2,000; cat. no. ab126785; abcam), rabbit anti‐CYP39a1 (1:1,000; cat. no. ab129334; abcam), rabbit anti‐isopentenyl‐diphos‐ phate δ isomerase 1 (iDi1; 1:3,000; cat. no. ab97448; abcam), rabbit anti‐lanosterol synthase [anti‐oxidosqualene‐lanosterol cyclase (oSC); 1:1,000; cat. no. ab80364; abcam], rabbit anti‐farnesyl diphosphate farnesyltransferase 1 (FDFT1; 1:1,000; cat. no. ab195046; abcam), rabbit anti‐farnesyl diphosphate synthase (FDPS; 1:2,000; cat. no. ab153805; Abcam), rabbit anti‐mevalonate diphosphate decarboxylase (MVD; 1:1,000; cat. no. ab96226; abcam), rabbit anti‐meva‐ lonate kinase (MVK; 1:1,000; cat. no. ab154515; abcam), rabbit anti‐3‐hydroxy‐3‐methylglutaryl‐CoA reductase (HMGCR; 1:1,000; cat. no. ab174830; abcam), rabbit anti‐low density lipoprotein receptor (lDlR; 1:1,000; cat. no. ab52818; abcam), rabbit anti‐c‐Jun n‐terminal kinase (JnK; 1:1,000; cat. no. 9252s; CST Biological Reagents Co., ltd.), mouse anti‐phosphorylated (p)‐JnK (1:2,000; cat. no. 9255s; CST Biological Reagents Co., Ltd.), rabbit anti‐p‐insulin receptor substrate 1 (p‐iRS1; Tyr608) mouse (1:1,000; cat. no. 09‐432; MilliporeSigma), rabbit anti‐iRS‐1 (1:1,000; cat. no. 2382S; CST Biological Reagents Co., Ltd.), rabbit anti‐p‐AKT (Ser473) (1:1,000; cat. no. 4060S; CST Biological Reagents Co., ltd.), rabbit anti‐aKT (1:1,000; cat. no. 4685S; CST Biological Reagents Co., ltd.), mouse anti‐tribbles pseudokinase 3 (TRB3; 1:100; cat. no. sc‐390242; Santa Cruz Biotechnology, inc.), rabbit anti‐c‐Jun (1:1,000; cat. no. ab32137; abcam), mouse anti‐p‐c‐Jun (1:200; cat. no. sc‐822; Santa Cruz Biotechnology, inc.) and goat anti‐KlB (0.5 μg/ml; cat. no. aF5889; R&D Systems China Co., Ltd.).



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